įunding: This study was completed through a participant guided, learn-by doing meta-research course funded by the Berlin University Alliance within the Excellence Strategy of the federal and state governments (301_TrainIndik, TLW).
Western blot results of fish code#
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: The abstraction protocols, search strategy, data, data visualization code and teaching slides are deposited in the Open Science Framework (OSF) repository (RRID:SCR_003238) at. Received: JAccepted: AugPublished: September 12, 2022Ĭopyright: © 2022 Kroon et al. PLoS Biol 20(9):Īcademic Editor: Mathieu J. (2022) Blind spots on western blots: Assessment of common problems in western blot figures and methods reporting with recommendations to improve them. Additional resources include a toolbox to help scientists produce more reproducible western blot data, teaching slides in English and Spanish, and an antibody reporting template.Ĭitation: Kroon C, Breuer L, Jones L, An J, Akan A, Mohamed Ali EA, et al. We present detailed descriptions and visual examples to help scientists, peer reviewers, and editors to publish more informative western blot figures and methods. Important antibody identifiers like company or supplier, catalog number, or RRID were omitted frequently for primary antibodies and regularly for secondary antibodies. Western blot methods sections often lack information on the amount of protein loaded on the gel, blocking steps, and antibody labeling protocol. Publishing blots with visible molecular weight markers is rare, and many blots additionally lack molecular weight labels. Our data show that most published western blots are cropped and blot source data are not made available to readers in the supplement. We systematically examined 551 articles published in the top 25% of journals in neurosciences ( n = 151) and cell biology ( n = 400) that contained western blot images, focusing on practices that may omit important information. While several groups have studied the prevalence of image manipulation or provided recommendations for improving western blotting, data on the prevalence of common publication practices are scarce. Unfortunately, poor western blot image display practices and a lack of detailed methods reporting can limit a reader’s ability to evaluate or reproduce western blot results. All rights reserved.Western blotting is a standard laboratory method used to detect proteins and assess their expression levels. These results demonstrate the usefulness of UI-25A as a tool to improve the understanding of antibody-mediated immunity in sablefish as well as to provide valuable information for vaccine development and expansion of aquaculture efforts for this fish species.Īeromonas salmonicida ELISA IgM Immunoglobulin Monoclonal antibody Sablefish Western blot.Ĭopyright © 2022 Elsevier Ltd. Immunofluorescent staining also demonstrated the ability of UI-25A to recognize membrane-bound IgM and identify IgM + cells in the head kidney. salmonicida recognized by this specific IgM from vaccinated sablefish. UI-25A was used in Western blot analyses to identify immunogenic regions of A. An ELISA was developed to measure Aeromonas salmonicida specific IgM in the plasma of sablefish from a previous experiment where fish were immunized with a proprietary A. Western blotting confirmed UI-25A recognizes the heavy chain of IgM and does not cross react to proteins or carbohydrates in serum of four other teleost species. In this paper, a monoclonal antibody, UI-25A, specific to sablefish IgM was produced in mice. There is limited information on both innate and adaptive immunity for this species and new tools are needed to determine antibody response following vaccination or disease outbreaks. Sablefish (Anoplopoma fimbria) are an emerging aquaculture species native to the continental shelf of the northern Pacific Ocean.
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